About
Polypeptide.ltd provides a full spectrum of high-quality peptide services, ranging from custom peptide synthesis (research and cGMP grade with regular and express deliveries) to high-throughput peptide library service. With access to state-of-the-art equipment and experienced chemists using the latest technologies, we have delivered over 118,000 custom peptides to more than 3,300 scientists worldwide. Now, we have become the first vendor in the peptide industry to be able to deliver peptides in as little as 5 days at 100% guaranteed quantity to meet the ever-increasing peptide needs in biological and drug discovery research.
Discovery Peptide Synthesis Service Highlights
Peptide Synthesis with Large Diversity: A wide choice for peptide synthesis includes
linear peptide (single SPPS length up to 70 AAs, ligation length up to 200 AAs), macrocyclic peptide, modified peptide and peptide based complex conjugates.
Excellent Quality & Quantity: We will provide the peptides to meet your requirements with purity ranging from crude to 99.9% & scale from mg to kg.
High Success Rate: Our professional technical team can ensure you more than a 98% success rate1 in peptide synthesis.
Quick Turnaround Time: Peptide synthesis with short timeline as fast as 3 days2 ensures your downstream research in a timely manner.
High-Throughput Peptide Synthesis: We have rich experience in synthesizing custom peptide libraries for peptide structure-activity-relationship (SAR) studies.
Comprehensive Analysis Support: We provide a default purity/MS profile and additional services including amino acid analysis, endotoxin analysis etc. after the completion of peptide synthesis.
Note:
1. All peptides in the 2022 projects with regular requests were successfully synthesized with the desired quality.
2. Turnaround time includes all business days from order confirmation to the completion of synthesis, not including shipping time. Turnaround time may be expected longer for the orders of over 60 peptides, or with rare modifications, salt exchange.
Linear Peptide Synthesis:
Approach:
Fmoc solid-phase peptide synthesis (SPPS)
Fragment Coupling
Ligation
Speed:
2-10 AA, single SPPS, 5-7 days
11-20 AA, single SPPS, 8-10 days
21-30 AA, single SPPS, 11-13 days
31-40 AA, single SPPS, 14-16 days
40-60 AA, single SPPS, 17-30 days
60-130 AA, conjugation/ligation, case by case
Cyclic Peptide Synthesis
Cyclization is now a common strategy for improving the pharmacological properties of a peptide from its linear precursor, including affinity and specificity, proteolytic stability, and/or solubility.
Our team has rich experience in cyclic peptide synthesis, with 100,000+ cyclic peptides successfully delivered.
Cyclization strategies include single/double/triple disulfide, click cyclization, lactone, lactam, RCM, etc. The cyclization positions can include head-to-tail, head-to-sidechain, sidechain-to-sidechain and sidechain-to-tail.
Experience in :
- Disulfide peptide (single/double/ triple disulfide);
- Thioether cyclization
- Lactam cyclization
- Lactone cyclization
- Click peptide
- RCM cyclization
- Bicyclic peptides
Bicyclo-disulfide bridge
(Three pairs of S-S bond formation achieved as well)
RCM monocyclic peptide
Bicyclic peptide
Click peptide
Cyclosporine analog
Thioether cyclization
Modified Peptide Synthesis
We offer a comprehensive range of special modifications to meet your needs. A few examples are shown below, including N-terminal modification, C-terminal modification, dye/fluorescent labeling, biotin labeling, PEGylation, post-translational modification, DOTA/NOTA labeling and isotope labeling.
Apart from sidechain and backbone modifications, we can also incorporate unnatural amino acids into the sequences. For unnatural amino acid catalog products, please visit here for details.
Modified Peptide Synthesis
N-terminal modification
C-terminal modification
Dye and Fluorescent (site specific modification)
PTMs (site specific modification)
Isotope labeling
Glycopeptide
H (free amine)
Acetylation
Fmoc
CBZ
Bz
Pyr
Biotinylation
Lipid tail
Dye and Fluorescent labeling
Automatic Peptide Synthesizer
For routine peptide synthesis, automatic synthesizers can accelerate the SPPS portion. Our synthesizers include CSBio, CEM Liberty Blue and Biotage Syro II. With these advanced synthesizers, as well as a variety of prep-HPLC equipment installed in house, your requested compound can be ready within as little as 5 days.
CEM Liberty Blue
As fast as 5 min/position
Microwave synthesizer
12-channel
Sequential synthesis
0.1-0.2 mmol
Biotage Syro II
High throughput synthesizer
24/48/96 channel
Parallel synthesis
Suitable for SAR screening
25 umol
CSBio Synthesizer
3-channel
Parallel synthesis
0.2-0.5 mmol
Symphony X
High throughput synthesizer
24 channel
Parallel synthesis
0.1-0.2 mmol